Isolation and purification of fungal pathogen (Macrophomina phaseolina) induced chitinase from moth beans (Phaseolus aconitifolius)
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چکیده
منابع مشابه
Product optimization, purification and characterization of a novel polygalacturonase produced by Macrophomina phaseolina
Introduction: Production of a novel polygalacturonase (PG) active at pH = 3.0 and suitable to be used in fruit juice industries from Macrophomina phaseolina was evaluated. Suitable carbon, nitrogen and phosphorous forms were determined and the condition was optimized for higher PG production. Materials and methods: Macrophomina phaseolina was cultured in so called production medium. The secreto...
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Macrophomina phaseolina has a wide host range and is responsible for causing losses on m cultivated and wild plant species. So far in Pakistan it has been reported to cause disease on hosts including field crops, pulses, flowers and vegetable have been reported. Infection on s first reported from Sri Lanka in 1927 and in 1982 it was reported from sunflower field in fungus is reported to be soil...
متن کاملPurification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans)
A lectin has been isolated from seeds of the Phaseolus vulgaris cv. "Anasazi beans" using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The lectin was comprised of two 30-kDa subunits with substantial N-terminal sequence similarity to other Phaseolu...
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A protein fraction which inhibits growth of weanling rats was obtained from Phaseolus vulgaris seeds of the Sanilac variety by extraction with sodium chloride solution and removal of extraneous materials by precipitation at pH 4.0 and absorption on I : I bentonite-Celite. The protein was precipitated by 0.75 saturation with ammonium sulfate. The growth inhibiting protein fraction contained at l...
متن کاملEvaluation of antifungal activity of Meliaceae family against Macrophomina phaseolina
Macrophomina phaseolina (Tassi) Goid is a soil-borne fungal pathogen that causes root rot disease in more than 500 plant species but no registered fungicide is available for its control. The present study was carried out to investigate the antifungal activity of aqueous leaf extracts of Azadirachta indica A. Juss., Melia azedarach L. and Toona ciliata Roxb. (Meliaceae) against M. phaseolina. Aq...
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ژورنال
عنوان ژورنال: Journal of Pharmacy And Bioallied Sciences
سال: 2010
ISSN: 0975-7406
DOI: 10.4103/0975-7406.62708